Futura


How the ABER biomass monitor works


▶ Real time means real savings

Cells with intact plasma membranes in a fermenter can be considered to act as tiny capacitors under the influence of an electric field. The non-conducting nature of the plasma membrane allows a build up of charge. The resulting capacitance can be measured: it is dependent upon the cell type and is directly proportional to the membrane bound volume of these viable cells. 


▶ A unique range of probes

- Choose from a range of in situ steam sterilisable probes manufactured in robust materials that conform to FDA requirements & USP Class VI. The smooth, annular ring electrode arrangement provides a surface free of areas of entrapment and large EVEN electric field; ideal for many cell culture applications where cells will collect in any well or crevice. For production applications ABER offers a highly robust 25mm probe with flush electrodes- a design that is often used in cGMP bioreactors for cell culture. ABER can also provide probes that can be gamma irradiated for single use bioreactors. Option for a range of probes for single use bioreactors including single use, sterilisable and flow through cell.


▶ Specification

Frequency Range:

50KHz to 20MHz

Measuring Ranges:

Capacitance: 0.0 to 400pF/cm
Conductivity: 1.0 to 40 mS/cm +/-0.1 mS/cm
(Higher ranges available with compatible probes)

Cell Concentration Range:

Depends on cell sizes but typically:
Yeast (6 µm): 106 cells/ml to 1010 Cells/ml
Bacteria (1 µm): 109 cells/ml to 1013 Cells/ml
Animal Cell (12 µm): 105 cells/ml to 109 Cells/ml
Plant Cell (50 µm): 103 cells/ml to 107 Cells/ml

Resolution:

Resolution: 0.1 pF/cm.
Approx:
Bacteria typically 0.1 g/L dry weight or 2x109 Cells/ml for E. Coli.
Yeast or Animal Cells 0.05g/L or 1 x105 Cells/ml
The relationship of these capacitance values to biomass levels depends upon the
cell type and line.

Accuracy:

Typically bett er than ± 3% or ± 2% of the reading

Stability:

Better than ± 0.2 pF/cm at constant temperature with standard conductivity solution of ~12 mS/cm

Linearity:

Better than ± 1% over 100 pF/cm

Precision:

Typically <±0.5 pF/cm, no filter active.

Power Supply:

Natively 24V DC. This is typically supplied by an Aber Hub running on 110V AC to
240V AC mains.

Environmental:

IP65 rated
Recommended ambient operating temperature range: 5°C to 40°C