EZ DNA Methylation-Direct Kits




● Highlights


▶ Complete bisulfite conversion of DNA directly from blood, soft tissue, cells, FFPE samples, and LCM samples. 

▶ Compatible with small sample inputs - as few as 10 cells or 50 pg DNA. 

▶ Desulphonation and recovery of bisulfite-treated DNA with a spin-column, 96-well plate, or magnetic beads. 

▶ Low fragmentation. 

▶ Includes Proteinase K for tissue digestion.



● Description


- The EZ DNA Methylation-DirectTM Kit is a further refinement of our popular EZ DNA Methylation and EZ DNA Methylation-Gold kits. The EZ DNA Methylation-Direct Kit features reliable and complete bisulfite conversion of DNA directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of these kits make it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.



● EZ DNA Methylation-Direct Bisulfite Chemistry


- EZ DNA Methylation-Direct" Kit bisulfite chemistry significantly improves C to U conversion kinetics. DNA was converted using either EZ DNA MethylationDirect or conventional bisulfite chemistries. Recovered DNA was amplified by PCR, then cloned, Sequences from individual clones were analyzed and quantitated. This data shows that EZ DNA Methylation Direct bisulfite chemistry improves the rate and extent > 99.8%) of C to U conversion of DNA as compared to conventional bisulfite chemistry.