ZymoPURE-Express Plasmid Midiprep Kit
● Highlights
▶ Innovative pellet-free procedure bypasses the standard cell-pelleting and resuspension steps. No large-scale centrifugation required!
▶ The fastest, easiest, most reliable method for purification of up to 1.2 mg of ultra pure endotoxin-free plasmid DNA.
▶ Plasmid DNA is eluted directly from a microcentrifuge column and is well-suited for transfection and other sensitive applications.
● Description
- The ZymoPURE-Express Plasmid Midiprep Kit is the fastest and easiest method for isolating plasmid DNA from E. coli. Utilizing a patented alkaline lysis method, this novel technology can purify up to 1.2 mg of high-quality endotoxin-free plasmid DNA directly from the culture, without pelleting and resuspension steps. Just add the ZymoPURE-Express Lysis Buffer directly to your bacterial culture, neutralize, filter debris, and purify using the Zymo-Spin V-P microcentrifuge column.
Innovative binding chemistry enables highly concentrated (up to 1.5 ug/ul) DNA to be eluted directly from microcentrifuge column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, and protein. Purified plasmid DNA is ideal for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
As an added convenience, the ZymoPURE-Express Plasmid Midiprep Kit contains patented colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization and syringe filters are included for rapid lysate clearing.
● Streamlined Pellet-Free Workflow
