Zyppy Plasmid Miniprep Kit




● HIGHLIGHTS


▶ Fastest: 8 minutes from culture flask to high-quality plasmid DNA.

▶ Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.

▶ High Quality: Ready for PCR, sequencing, cloning, and transfection.



● DESCRIPTION


- The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.



● Technical Specifications 


Applicable For

Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.

Elution Volume

≥ 30 µl

Equipment

Microcentrifuge and/or a vacuum manifold.

Processing Time

8 min

Purity

Typical Abs260/280 ≥1.8.

Size Range

Up to 25 kb

Yield

Up to 25 µg of plasmid DNA per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.



● Product FAQ


Q1: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?

- Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.


Q2: I accidently left my Neutralization Buffer at room temperature. Will it be okay?

- Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible.


Q3: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?

- ≤ 50 Endotoxin Units/µg of plasmid DNA.


Q4: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?

- No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.


Q5: Can the purified plasmid be transfected into eukaryotic cell lines?

- Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.


Q6: What is the composition of the Zyppy Elution buffer?

- 10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.


Q7: Can E.coli grown in enriched growth media be used with this kit?

- Yes, pellet and resuspend E.coli in water or TE before proceeding with the protocol.




● Technology Overview: Zyppy® Pellet-free Procedure


▶ Plasmid DNA isolation directly from culture

- The Zyppy Plasmid Miniprep Kit features a pellet-free, modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify using provided Zymo-Spin Column technology. Additionally, the innovative colored buffers included in the kit permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The plasmid DNA is of the highest quality and ready for sensitive downstream applications.






● High-throughput and Automated Plasmid DNA Purification

- The Zyppy pellet-free procedure from Zymo Research allows for fully automated, highthroughput plasmid purification. No centrifugation or re-suspension steps common to all other conventional procedures are required. The kit features a modified alkaline lysis system that allows for the direct lysis of E. coli in the growth medium. With Zyppy's easy, pellet-free procedure, you can grow, lyse, and process samples in the same plate with no manual manipulation.

Samples grown overnight in a 96-well block are transferred to an automated liquid handler (e.g., Tecan - Freedom Evo®). The uniquely formulated Deep Blue Lysis Buffer is added directly to bacterial cultures in each well. After neutralization, lysate separation steps are expedited using innovative MagClearing Beads to pull down cellular debris. The cleared lysates are then automatically transferred to another plate and MagBinding Beads are added to the cleared lysate and the DNA-bound beads are washed and dried. Once eluted, plasmid DNA is ready for immediate use, or can be stored at -20°C for later use.



● Zyppy® Plasmid Kits

▶ Highlights
- The fastest, easiest kits available for high-throughput transfection-quality plasmid DNA purification. Pellet-free procedure omits conventional cell pelleting and resuspension steps. DNA quality appropriate for cloning, sequencing, and transfection.


▶ Description
- The Zyppy Plasmid Kits feature a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and purify using the provided Zymo-Spin Column technology. Additionally, the innovative colored buffers included in the kit enable error-free visualization and identification of complete bacterial cell lysis and neutralization.

The Zyppy Plasmid Kits are available in a variety of sizes to fit your research needs. Our miniprep's binding capacity is up to 25 pg of DNA per prep, the 96-well miniprep's binding capacity is 10 ug per prep, and our midiprep's binding capacity is up to 120 ug per prep.

The Zyppy Plasmid Kits are some of the fastest and easiest methods available to separate plasmid DNA from E. coli efficiently. The plasmid DNA is of the highest quality, endotoxinfree, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.


▶ Streamlined Workflow