Direct-zol-96 MagBead RNA
● HIGHLIGHTS
▶ Easy Handling: Bypass chloroform, phase separation and precipitation steps.
▶ NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
▶ Non-Biased: Complete RNA recovery without miRNA loss.
● DESCRIPTION
- The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well), magnetic bead-based isolation of high-quality RNA directly from samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small/microRNAs (≥17 nt) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-PCR, transcription profiling, hybridization, etc.
● Technical Specifications
Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. |
|---|---|
Equipment | Microcentrifuge, vortex, magstand |
Sample Inactivation | TRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents. |
Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
Size Range | Total RNA ≥ 17 nt |
Yield | 10 µg RNA (binding capacity), ≥ 30 µl (elution volume) |
