Quick-RNA Miniprep Plus Kit
● HIGHLIGHTS
▶ Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
▶ DNA-Free: Genomic DNA removal column and DNase I included.
▶ NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
● DESCRIPTION
- The Quick-RNA Miniprep Plus Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from cells, all tissue types, whole blood, and biological fluids. The provided DNA/RNA Shield stabilizes samples, allowing them to be stored without the need for immediate freezing or processing for up to one month. Furthermore, DNA/RNA Shield™ inactivates RNases as well as microbial pathogens (viruses, bacteria, etc.). The procedure combines a unique buffer system with Zymo-Spin Column technology to yield high quality total RNA (including small RNAs 17-200 nt). Simply add DNA/RNA Shield and Proteinase K to extract total RNA from any tissue, then purify the RNA using the Zymo-Spin Column. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing, etc. In addition, the kit can be used for the enrichment of small and large RNAs in two separate fractions.
● Technical Specifications
Equipment | Microcentrifuge, vortex, heat block/bath (55ºC) |
|---|---|
Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
Sample Source | Any cells (animal, blood cells etc.), all tissues (tough-to-lyse, FFPE, etc.), blood, biological fluids, and enzymatic reactions (e.g., DNase I), samples in DNA/RNA Shield™, TRIzol® or RNAlater™. |
Size Range | Total RNA ≥ 17 nt |
Yield | spin column, 100 µg RNA (binding capacity), ≥50 µl (elution volume) |
