Mix & Go! Competent Cells - DH5 Alpha
● HIGHLIGHTS
▶ Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
▶ High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
▶ Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.
● DESCRIPTION
- The Mix & Go! Competent Cells - DH5 Alpha are premade E. coli DH5 alpha competent cells for simple and highly efficient DNA transformation. These DH5 alpha competent cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
● Technical Specifications
Additional Info | Insert stability due to recA1 mutation. Can be used for blue/white screening, accepts large plasmids due to deoR mutation. High plasmid yield due to endA1 mutation. |
|---|---|
Genotype | F-Φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1,λ- |
Processing Time | 20 Seconds |
Product Storage | -70°C to -80°C |
Transformation Efficiency | 108 - 109 transformants per µg of plasmid DNA |
